MersV1, France, Analysis, bibRecord, 000294

A pseudoknotted tRNA variant is a substrate for tRNA (cytosine-5)-methyltransferase from Xenopus laevis

Identifieur interne : 000294 ( France/Analysis ); précédent : 000293; suivant : 000295

A pseudoknotted tRNA variant is a substrate for tRNA (cytosine-5)-methyltransferase from Xenopus laevis

Auteurs : Hervé Brulé [France] ; Henri Grosjean [France] ; Richard Giegé [France] ; Catherine Florentz [France]

Source :

Biochimie [ 0300-9084 ] ; 1998.

RBID : ISTEX:02400B0AE40FC063AB70C507AC2E17A605294A4D

English descriptors

KwdEn :
- TLC, TYMV, Xenopus laevis, cps, methylase, post-transcriptional modification, tRNA, tertiary structure, turnip yellow mosaic virus.
Teeft :
- Acceptor, Acceptor branch, Anticodon, Biochimie, Canonical, Canonical trnas, Coli, Denaturing polyacrylamide, Elution solution, Enzymatic formation, Enzyme, Eukaryotic trna, Gieg6, Grosjean, Laevis, Methylation, Microinjected, Modification, Modification pattern, Mosaic, Mosaic virus, Nuclease, Nucleic, Nucleic acids, Nucleotide, Oocyte, Potential target nucleotides, Press washington, Rna, Rnase, Structural features, Transcript, Trna, Turnip, Tymv, Tymv domain, Tymv structure, Viral, Xenopus, Xenopus laevis, Xenopus laevis oocyte, Xenopus laevis oocytes, Xenopus oocytes, Yeast, Yeast trna, Yeast trnas.

Abstract

Abstract: tRNA post-transcriptional modification enzymes of Xenopus laevis were proposed previously to belong to two major groups according to their sensitivity to structural perturbations in their substrates. To further investigate the structural variations tolerated by these enzymes, the tRNA-like domain of turnip yellow mosaic virus RNA (88 nucleotides in length) has been microinjected into the oocytes of Xenopus laevis. This RNA possesses 12 potential target nucleotides for modification within a structure including a pseudoknotted folding, an extended anticodon stem, and unusual D-loop/T-loop interactions. Results indicate that only cytosine-42, a position equivalent to C-49 in canonical tRNAs, was quantitatively modified into m5C in the microinjected RNA. Modification was detected to high levels, indicating that at least one enzyme tolerates non-canonical structural features.

Url:

https://api.istex.fr/ark:/67375/6H6-8WH36MQS-D/fulltext.pdf

DOI: 10.1016/S0300-9084(99)80003-0

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000541
to stream Istex, to step Curation: 000541
to stream Istex, to step Checkpoint: 001404
to stream Main, to step Merge: 003C44
to stream Main, to step Curation: 003B94
to stream Main, to step Exploration: 003B94
to stream France, to step Extraction: 000294

Links to Exploration step

ISTEX:02400B0AE40FC063AB70C507AC2E17A605294A4D

Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: tRNA post-transcriptional modification enzymes of Xenopus laevis were proposed previously to belong to two major groups according to their sensitivity to structural perturbations in their substrates. To further investigate the structural variations tolerated by these enzymes, the tRNA-like domain of turnip yellow mosaic virus RNA (88 nucleotides in length) has been microinjected into the oocytes of Xenopus laevis. This RNA possesses 12 potential target nucleotides for modification within a structure including a pseudoknotted folding, an extended anticodon stem, and unusual D-loop/T-loop interactions. Results indicate that only cytosine-42, a position equivalent to C-49 in canonical tRNAs, was quantitatively modified into m5C in the microinjected RNA. Modification was detected to high levels, indicating that at least one enzyme tolerates non-canonical structural features.</div>
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A pseudoknotted tRNA variant is a substrate for tRNA (cytosine-5)-methyltransferase from Xenopus laevis

A pseudoknotted tRNA variant is a substrate for tRNA (cytosine-5)-methyltransferase from Xenopus laevis

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri